Establishment and preliminary application of a rapid fluorescent focus inhibition test (RFFIT) for rabies virus
نویسندگان
چکیده
منابع مشابه
Comparison of a Micro-Neutralization Test with the Rapid Fluorescent Focus Inhibition Test for Measuring Rabies Virus Neutralizing Antibodies.
The rapid fluorescent focus inhibition test (RFFIT) is routinely used in the United States to measure rabies virus neutralizing antibodies (rVNA). RFFIT has a long history of reproducible and reliable results. The test has been modified over the years to use smaller volumes of reagents and samples, but requires a 50 μL minimum volume of test serum. To conduct pathogenesis studies, small laborat...
متن کاملComparison of the detection and quantification of rabies antibodies in canine sera
Rabies antibodies in canine sera were detected and quantified by methods: virus neutralisation test on mice (VNT), rapid fluorescence focus inhibition test (RFFIT), and fluorescent antibody virus neutralisation (FAVN). e results of rabies antibodies levels in non-vaccinated dogs obtained by all three methods were in correlation. e comparison of rabies antibody titres determined in vaccinated ...
متن کاملInactivation of rabies virus in reagents used for the fluorescent rabies antibody test.
Procedures for inactivating rabies virus in reagents used for the fluorescent rabies antibody test are described. Mouse brain adsorbing suspensions containing greater than or equal to 10(9) 50% lethal doses of virus per ml were rendered noninfectious by treatment with 0.1% beta-propiolactone or by heating at 56 degrees for greater than or equal to 30 min. Viable virus in tissue impression smear...
متن کاملEfficacy of Purified Vero Cell Rabies Vaccine (PVRV) under the Zagreb Regimen in Iran
Background: Despite the effective pre- and post-exposure treatments, at least 60,000 deaths from rabies occur worldwide every year. Post-exposure treatment is considered as one of the most significant measures for preventing human deaths in exposed individuals. The 2-1-1 rabies post-exposure treatment schedule known as Zagreb regimen is an abbreviated immunization plan in which a tissue cultu...
متن کاملImmunogenicity of BHK-Rabies Vaccine in Cattle
In this study, the immunogenicity of a rabies vaccine that was used in one of the Isfahan cattle farms is reported. This vaccine was produced by replication of the Pasteur virus (PV) strain on BHK-21 monolayer cell culture. The virus suspension was inactivated by ß-propiolactone treatment and the harvested pool showed sterility and safety. Thus, the obtained vaccine was tested on mice and a sat...
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ژورنال
عنوان ژورنال: Virologica Sinica
سال: 2013
ISSN: 1674-0769,1995-820X
DOI: 10.1007/s12250-013-3321-x